Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

沂水崔家峪玻璃用石英砂岩矿地质特征

沂水崔家峪玻璃用石英砂岩矿床赋存于早寒武世李官组砂岩段中．呈近水平的层状产出。因其岩石坚硬．矿体呈环山的平台状分布。矿体厚度大，矿石品级高，特级品矿石二氧化硅平均含量98．47％，铁杂质平均含量0．043％(选矿后，铁杂质含量可降至0．02％以下)，为一优质玻璃硅质原料矿床。矿石为细一中粒石英砂岩，粒度以中粒为主，矿石由碎屑颗粒和胶结物组成，碎屑成分含量为97％～98％．其中绝大部分是石英颗粒．具典型的砂屑结掏。该矿床属滨海陆源沉积矿床。     

SDSS J143030.22-001115.1： A Misclassified Narrow-line Seyfert 1 Galaxy with Flat X-ray Spectrum

We used multi-component profiles to model the Hβ and [OⅢ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy (NLS1) in a sample of 150 NLS1 candidates selected from the Sloan Digital Sky Survey (SDSS), Early Data Release (EDR). After subtracting the Hβ contribution from narrow line regions (NLRs), we found that its full width half maximum (FWHM) of broad Hβ line is nearly 2900km s~(-1) , significantly larger than the customarily adopted criterion of 2000 km s~(-1) . With its weak Fe Ⅱ multiples, we believe that SDSS J143030.22-001115.1 should not be classified as a genuine NLS1. When we calculate the virial black hole masses of NLS1s, we should use the Hβ linewidth after subtracting the NLR component.     

Multistep matched-field inversion for broad-band data from ASIAEX2001

This paper discusses the results of geoacoustic inversion carried out using explosive charge data from the Asian Seas International Acoustic Experiment (ASIAEX) East China Sea (ECS) Experiment. A multifrequency incoherent matched-field inversion processor and a genetic algorithm (GA) are used for the inversion. A multistep matched field inversion approach is presented, which makes use of the varying sensitivities of wave fields at various frequencies to reduce the inversion problem into a sequence of smaller inversions with fewer unknowns to estimate at each stage. Different parameters are estimated using data at different frequencies according to their sensitivities. Inversion results for different areas in the ECS region are summarized and compared with core data.     

毫秒级快速尖峰辐射事件的时间和频率特性

介绍了太阳22周峰年期间云南天文台米波射电频谱仪的观测结果。主要对1557个单个Spike的时间和频率分布作了统计分析，着重指出太阳快速精细结构的观测特征并定性地指出其产生机制。     

5个新发现的X选BL Lac天体

利用ＲＯＳＡＴＶＬＡ方法筛选，从ＲＯＳＡＴＸ射线源中选出了一批新的ＢＬＬａｃ天体和类星体的候选体．１９９６年１２月８日至１７日，利用北京天文台２．１６ｍ望远镜和新从美国引进的ＯＭＲ摄谱仪，对这批新候选体进行了光谱认证．经ＳＵＮ工作站处理，又发现了５个新的Ｘ选ＢＬＬａｃ天体．此外，还对去年作者在ＯＭＲ引进之前发现的ＢＬＬａｃ天体进行了检验，结果发现，去年发现的７个ＢＬＬａｃ天体中，２３２２＋３４３的ＣａＩＩＨ＆Ｋ“Ｂｒｅａｋｓｔｒｅｎｇｔｈ”为２６．９％，稍大于判据２５％，因此，２３２２＋３４３到底是ＢＬＬａｃ天体还是射电星系还有待于进一步测光及偏振观测的验证．     

Theory of stability, and regulation and control of ecological system in oasis

Starting with analysis on the evolving course of oasis and the characteristics and evolution of transitional zone between oasis and desert, in consideration of ecological elements including plant stomata resistance, area covered by vegetation, and physical elements including albedo of vegetation and bare soil, atmosphere temperature, and humidity, under the condition of the balance among net radiation flux, latent heat flux, and sensible heat flux, the following are calculated: temperatures of vegetation and bare soil in different conditions, as well as the evapotranspiration rate of ecosystem. Analysis on evapotranspiration rate indicates that it depends on both the climate of environment and the physiological and ecological conditions of plants. On certain conditions, the evapotranspiration rate of transitional zone between oasis and desert (i.e. area covered by vegetation less than 20%), in some parameter domains, appears in bifurcation or multiequilibrium state. Meanwhile, in such area, ecosystem is extremely unstable. Any minor change to the balance will cause either increase or reduction of area covered by vegetation in ecosystem, on the basis of discussion on the emergency of these phenomena. This paper is attempting to propose an effective way of destruction and rebuilt ecosystem in transitional zone. The way is to control the evaporation of plant through selecting anti-drought country plant with big stomata resistance, and modify the roughness of the underlying surface in ecosystem by establishing rational interspace structure of plant community, so as to put the degenerative ecosystem into the natural succession track. This primary theory is being verified through observation and analysis on historical data.     

澳门附近海域沉积物污染状况及特征分析

掌握澳门附近海域沉积物的污染状况及特征,对于合理安排澳门海洋海岸工程疏浚泥的海洋倾倒,防止造成海洋污染有十分重要的意义。根据1997—1999年连续三年对澳门附近主要区域的海洋沉积物采样测定结果,通过分析认为澳门附近海域沉积物污染具有下列特征:1)沉积物中重金属和油类含量普遍较高,除了总Hg外均高于珠江口海区的其他港口;2)沉积物中污染物的分布受人类活动的影响明显,澳门内港受船只排污、码头作业、陆地工业和生活污水排放的影响,是澳门附近海域沉积物污染最为严重区域;3)受水质交换的影响明显,澳门外港、路环岛东北等区域受珠江径流影响水质交换较快,沉积物中污染物的含量较低,而内港由于水质交换较差,沉积物中污染物的含量相对较高;4)与沉积物的成分组成(吸附)有关,大部分污染物与沉积物中粉砂有较好的相关性,表明粉砂对污染物质有较广泛性的吸附能力,尤其是Cu、Zn、Cd、油类、有机质。     

火焰发射光谱法测定氯化物型湖卤水中钾和钠

氯化物型湖卤水中一般呈现高钠低钾的分布状态,实验配制了合理的钾钠分布比例的标准系列,原水样不需特殊处理,通过逐级稀释,采用最高标准调节能量,火焰发射光谱法直接测定湖卤水中的钾和钠,在标准曲线上查得被测离子的质量,然后换算成钾和钠的含量.方法用于氯化物型湖卤水中钾和钠的测定,回收率为91%～116%,精密度(RSD,n=7)小于1%.     

西藏驱龙斑岩铜钼矿床中UST石英的发现:初始岩浆流体的直接记录

单向固结结构(UST)是浅成侵位的岩浆出溶过程中形成的一种特殊结构,一般由梳状石英与细晶(斑岩)岩交互生长而成,少数产于斑岩与围岩接触部位,其内的原生包裹体被认为是初始流体出溶的可靠记录.作者在西藏驱龙铜矿床中首次发现了具有单向固结结构的石英.研究表明,驱龙UST石英存在于后期侵位的二长花岗斑岩与花岗闪长岩的接触部位,部分为高温β石英;UST石英中原生包裹体的成分主要为高盐度液相,除石盐子矿物外,还含有硬石膏等其他子矿物.阴极发光及显微测温结果表明,初始流体的出溶发生在高温(t≥573℃)、高压(P≥150～200 MPa)条件下,出溶的流体为高温、高盐度[w(NaCkeq)为44.5%～58%]流体,同时还具有较高的氧逸度.因形成压力较高,判断UST石英不可能由较浅侵位的二长花岗斑岩岩枝冷凝出溶而形成,从而推测驱龙铜矿床深部存在着孕育成矿斑岩的大型岩基.